产品名称
Mouse TIMP2 ELISA Kit
指标别名
CSC 21K; Metalloproteinase inhibitor 2; TIMP 2; TIMP2; TIMP-2
免疫原
Expression system for standard: NS0; Immunogen sequence: C27-P220
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Metalloproteinase inhibitor 2
文献引用格式
Mouse TIMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0955)
背景资料
TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0955-CAP | 96-well plate precoated with anti- mouse TIMP-2 antibody | 1 |
EK0955-ST | Lyophilized recombinant mouse TIMP-2 standard | 10ng/tube×2 |
EK0955-DA | Biotinylated anti- mouse TIMP-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 193 | 1314 | 4248 | 179 | 1283 | 4028 |
Standard deviation | 10.42 | 67.01 | 318.6 | 10.91 | 84.67 | 370.57 |
CV(%) | 5.4% | 5.1% | 7.5% | 6.1% | 6.6% | 9.2% |
典型数据示例
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.018 | 0.054 | 0.105 | 0.189 | 0.458 | 0.759 | 1.468 | 2.267 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)