产品名称
Human IL20 ELISA Kit
指标别名
Cytokine Zcyto10; IL 20; IL20; interleukin 20; ZCYTO10; IL-20
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human IL20 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0599)
背景资料
Interleukin-20(IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0599-CAP | 96-well plate precoated with anti- human IL-20 antibody | 1 |
| EK0599-ST | Lyophilized recombinant human IL-20 standard | 10ng/tubex2 |
| EK0599-DA | Biotinylated anti- human IL-20 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 97 | 693 | 1747 | 90 | 624 | 1759 |
| Standard deviation | 5.72 | 47.81 | 132.77 | 6.12 | 44.92 | 138.96 |
| CV(%) | 5.9 | 6.9 | 7.6 | 6.8 | 7.2 | 7.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.008 | 0.041 | 0.088 | 0.164 | 0.298 | 0.579 | 1.037 | 1.859 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)