产品名称
Mouse IL20 ELISA Kit
指标别名
Cytokine Zcyto10; IL 20; IL20; interleukin 20; ZCYTO10; IL-20
免疫原
Expression system for standard: E.coli; Immunogen sequence: L25-E176
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL20 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1350)
背景资料
IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1350-CAP | 96-well plate precoated with anti- mouse IL-20 antibody | 1 |
| EK1350-ST | Lyophilized recombinant mouse IL-20 standard | 10ng/tube×2 |
| EK1350-DA | Biotinylated anti- mouse IL-20 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 93 | 232 | 997 | 88 | 234 | 940 |
| Standard deviation | 6.41 | 11.6 | 66.79 | 6.86 | 14.5 | 68.62 |
| CV(%) | 6.9% | 5% | 6.7% | 7.8% | 6.2% | 7.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.01 | 0.039 | 0.069 | 0.137 | 0.276 | 0.612 | 1.222 | 2.319 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)