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Human CA9 ELISA Kit

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EK0682

  • 96T ¥2480
  • 货期: 现货
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产品名称
Human CA9 ELISA Kit
规格/价格
96T|2480元
指标别名
CA IX; CA9; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; carbonic anhydrase IX; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated antigen G250
免疫原
NSO, P59-D414
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
CA9
基因名全称
carbonic anhydrase IX
Uniprot ID
Q16790  
文献引用格式
Human CA9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0682)
背景资料
Carbonic anhydrase 9(CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
显色时间
20-25分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0682-CAP96-well plate precoated with anti- human CA9 antibody1
EK0682-STLyophilized recombinant human CA9 standard10ng/tubex2
EK0682-DABiotinylated anti- human CA9 antibody100ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)100ul(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3719641233176428
Standard deviation2.8410.9725.132.7711.6129.96
CV(%)7.75.66.18.46.67
典型数据示例
Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0890.1810.250.3680.6020.9841.5752.126
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine
实验时长
3.5h

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