指标别名
CA IX; CA9; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; carbonic anhydrase IX; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated antigen G250
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
carbonic anhydrase IX
文献引用格式
Human CA9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0682)
背景资料
Carbonic anhydrase 9(CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0682-CAP | 96-well plate precoated with anti- human CA9 antibody | 1 |
| EK0682-ST | Lyophilized recombinant human CA9 standard | 10ng/tubex2 |
| EK0682-DA | Biotinylated anti- human CA9 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 37 | 196 | 412 | 33 | 176 | 428 |
| Standard deviation | 2.84 | 10.97 | 25.13 | 2.77 | 11.61 | 29.96 |
| CV(%) | 7.7 | 5.6 | 6.1 | 8.4 | 6.6 | 7 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.089 | 0.181 | 0.25 | 0.368 | 0.602 | 0.984 | 1.575 | 2.126 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine