指标别名
CA IX; CA9; CAIX; Carbonate dehydratase IX; Carbonic anhydrase 9; carbonic anhydrase IX; G250; Membrane antigen MN; MN; P54/58N; pMW1; RCC associated antigen G250
免疫原
Expression system for standard: NS0; Immunogen sequence: Q32-D390
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Carbonic anhydrase 9
文献引用格式
Mouse CA9 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1587)
背景资料
Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1587-CAP | 96-well plate precoated with anti- mouse CA9 antibody | 1 |
| EK1587-ST | Lyophilized recombinant mouse CA9 standard | 10ng/tube×2 |
| EK1587-DA | Biotinylated anti- mouse CA9 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 34 | 186 | 448 | 35 | 180 | 461 |
| Standard deviation | 2.55 | 12.27 | 28.67 | 2.73 | 13.32 | 35.49 |
| CV(%) | 7.5% | 6.6% | 6.4% | 7.8% | 7.4% | 7.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.053 | 0.115 | 0.176 | 0.285 | 0.476 | 0.849 | 1.549 | 2.374 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine