产品名称
Human IL17F ELISA Kit
指标别名
Cytokine ML 1; IL 17F; IL17F; IL-17F; interleukin 17F; ML 1; ML1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human IL17F ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0795)
背景资料
Members of the interleukin-17 family, such as IL17F, are involved in the regulation of normal versus aberrant T-cell responses.. It is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0795-CAP | 96-well plate precoated with anti- human IL-17F antibody | 1 |
| EK0795-ST | Lyophilized recombinant human IL-17F standard | 10ng/tube×2 |
| EK0795-DA | Biotinylated anti- human IL-17F antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 121 | 432 | 1856 | 123 | 462 | 1927 |
| Standard deviation | 7.50 | 28.94 | 83.52 | 8.49 | 32.80 | 140.67 |
| CV(%) | 6.2 | 6.7 | 4.5 | 6.9 | 7.1 | 7.3 |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.035 | 0.055 | 0.103 | 0.162 | 0.305 | 0.627 | 1.201 | 1.992 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin or EDTA)