指标别名
Cytokine ML 1; IL 17F; IL17F; IL-17F; interleukin 17F; ML 1; ML1
免疫原
Expression system for standard: E.coli; Immunogen sequence: A19-A153
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat IL17F ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1136)
背景资料
Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1136-CAP | 96-well plate precoated with anti- rat IL-17F antibody | 1 |
| EK1136-ST | Lyophilized recombinant rat IL-17F standard | 10ng/tube×2 |
| EK1136-DA | Biotinylated anti- rat IL-17F antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 57 | 188 | 966 | 55 | 186 | 917 |
| Standard deviation | 3.3 | 10.15 | 41.53 | 3.96 | 12.46 | 52.26 |
| CV(%) | 5.8% | 5.4% | 4.3% | 7.2% | 6.7% | 5.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.012 | 0.126 | 0.206 | 0.328 | 0.554 | 0.923 | 1.516 | 2.371 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)