产品名称
Human RANK/TNFRSF11A ELISA Kit
指标别名
TNF receptor superfamily member 11a; CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER; TNFRSF11A
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
文献引用格式
Human RANK/TNFRSF11A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0829)
背景资料
Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0829-CAP | 96-well plate precoated with anti- human RANK antibody | 1 |
| EK0829-ST | Lyophilized recombinant human RANK standard | 10ng/tube×2 |
| EK0829-DA | Biotinylated anti- human RANK antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 87 | 619 | 1237 | 91 | 558 | 1284 |
| Standard deviation | 3.91 | 40.23 | 58.13 | 5.36 | 39.61 | 84.74 |
| CV(%) | 4.5 | 6.5 | 4.7 | 5.9 | 7.1 | 6.6 |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.007 | 0.155 | 0.284 | 0.476 | 0.806 | 1.313 | 1.818 | 2.166 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum
联系我们
关注我们
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
