产品名称
Mouse RANK/TNFRSF11A ELISA Kit
指标别名
TNF receptor superfamily member 11a; CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER; Tnfrsf11a
免疫原
Expression system for standard: NS0; Immunogen sequence: Q30-P213
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Tumor necrosis factor receptor superfamily member 11A
文献引用格式
Mouse RANK/TNFRSF11A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0830)
背景资料
Receptor Activator of Nuclear Factor kappa B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0830-CAP | 96-well plate precoated with anti- mouse RANK antibody | 1 |
| EK0830-ST | Lyophilized recombinant mouse RANK standard | 10ng/tube×2 |
| EK0830-DA | Biotinylated anti- mouse RANK antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 210 | 719 | 1032 | 218 | 725 | 978 |
| Standard deviation | 11.97 | 30.19 | 46.44 | 16.35 | 42.05 | 53.79 |
| CV(%) | 5.7% | 4.2% | 4.5% | 7.5% | 5.8% | 5.5% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.042 | 0.092 | 0.121 | 0.194 | 0.319 | 0.57 | 1.023 | 2.01 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum