产品名称
Rat RANK/TNFRSF11A ELISA Kit
指标别名
TNF receptor superfamily member 11a; CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TRANCER; TNFRSF11A
免疫原
Expression system for standard: NS0; Immunogen sequence: Q30-P213
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
tumor necrosis factor receptor superfamily, member 11a, NFKB activator
文献引用格式
Rat RANK/TNFRSF11A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1281)
背景资料
Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1281-CAP | 96-well plate precoated with anti- rat RANK antibody | 1 |
| EK1281-ST | Lyophilized recombinant rat RANK standard | 10ng/tube×2 |
| EK1281-DA | Biotinylated anti- rat RANK antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 209 | 514 | 1401 | 226 | 542 | 1518 |
| Standard deviation | 13.58 | 34.95 | 88.26 | 18.3 | 42.81 | 97.15 |
| CV(%) | 6.5% | 6.8% | 6.3% | 8.1% | 7.9% | 6.4% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.067 | 0.125 | 0.175 | 0.25 | 0.45 | 0.848 | 1.489 | 2.053 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants and Serum
联系我们
关注我们
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
