产品名称
Mouse KIM-1/HAVCR1 ELISA Kit
指标别名
hepatitis A virus cellular receptor 1; CD365; HAVCR; HAVCR-1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; HAVCR1
免疫原
Expression system for standard: NS0; Immunogen sequence: Y22-T212
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
hepatitis A virus cellular receptor 1
文献引用格式
Mouse KIM-1/HAVCR1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0880)
背景资料
KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0880-CAP | 96-well plate precoated with anti- mouse KIM1 antibody | 1 |
EK0880-ST | Lyophilized recombinant mouse KIM1 standard | 10ng/tube×2 |
EK0880-DA | Biotinylated anti- mouse KIM1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 87 | 279 | 842 | 85 | 261 | 792 |
Standard deviation | 6.87 | 14.78 | 36.2 | 7.31 | 14.87 | 38.8 |
CV(%) | 7.9% | 5.3% | 4.3% | 8.6% | 5.7% | 4.9% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.028 | 0.074 | 0.114 | 0.227 | 0.422 | 0.869 | 1.481 | 2.222 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine