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Mouse KIM-1/HAVCR1 ELISA Kit

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EK0880

  • 96T ¥2480
  • 货期: 现货

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产品名称
Mouse KIM-1/HAVCR1 ELISA Kit
规格/价格
96T/2480
指标别名
hepatitis A virus cellular receptor 1; CD365; HAVCR; HAVCR-1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; HAVCR1
免疫原
Expression system for standard: NS0; Immunogen sequence: Y22-T212
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
HAVCR1
基因名全称
hepatitis A virus cellular receptor 1
Uniprot ID
Q3V033  
文献引用格式
Mouse KIM-1/HAVCR1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0880)
背景资料
KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
试剂盒内容
Catalog numberDescriptionQuantity
EK0880-CAP96-well plate precoated with anti- mouse KIM1 antibody1
EK0880-STLyophilized recombinant mouse KIM1 standard10ng/tube×2
EK0880-DABiotinylated anti- mouse KIM1 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)8727984285261792
Standard deviation6.8714.7836.27.3114.8738.8
CV(%)7.9%5.3%4.3%8.6%5.7%4.9%
典型数据示例
Concentration(pg/ml)031.262.512525050010002000
O.D.0.0280.0740.1140.2270.4220.8691.4812.222
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine
实验时长
3.5h

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