产品名称
Rat KIM-1/HAVCR1 ELISA Kit
指标别名
hepatitis A virus cellular receptor 1; CD365; HAVCR; HAVCR-1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1
免疫原
Expression system for standard: NS0; Immunogen sequence: S18-V238
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
hepatitis A virus cellular receptor 1
文献引用格式
Rat KIM-1/HAVCR1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0882)
背景资料
KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2.
试剂盒内容
Catalog number | Description | Quantity |
EK0882-CAP | 96-well plate precoated with anti- rat KIM1 antibody | 1 |
EK0882-ST | Lyophilized recombinant rat KIM1 standard | 10ng/tube×2 |
EK0882-DA | Biotinylated anti- rat KIM1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 87 | 272 | 938 | 86 | 293 | 1026 |
Standard deviation | 3.82 | 20.67 | 59.09 | 3.95 | 23.14 | 84.13 |
CV(%) | 4.4% | 7.6% | 6.3% | 4.6% | 7.9% | 8.2% |
典型数据示例
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.007 | 0.052 | 0.081 | 0.212 | 0.386 | 0.740 | 1.331 | 1.973 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine