产品名称
Human KIM-1/HAVCR1 ELISA Kit
指标别名
hepatitis A virus cellular receptor 1; CD365; HAVCR; HAVCR-1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1; HAVCR1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
hepatitis A virus cellular receptor 1
文献引用格式
Human KIM-1/HAVCR1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0883)
背景资料
KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rats is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to Rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the Rat Tim1 gene and database analysis, the HAVCR1 gene was mapped to 5q33.2.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0883-CAP | 96-well plate precoated with anti- human KIM1 antibody | 1 |
| EK0883-ST | Lyophilized recombinant human KIM1 standard | 10ng/tube×2 |
| EK0883-DA | Biotinylated anti- human KIM1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 92 | 215 | 880 | 93 | 208 | 802 |
| Standard deviation | 5.42 | 9.24 | 39.6 | 5.48 | 11.02 | 40.1 |
| CV(%) | 5.9 | 4.3 | 4.5 | 5.9 | 5.3 | 5 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.045 | 0.127 | 0.194 | 0.301 | 0.506 | 0.935 | 1.57 | 2.607 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum, Plasma (heparin, EDTA) and Urine