Human Endostatin/COL18A1 ELISA Kit

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EK0886

96T ￥2480
货期：现货

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产品名称

Human Endostatin/COL18A1 ELISA Kit

规格/价格

96T|2480

指标别名

COL18A1; Collagen alpha 1(XVIII) chain; collagen; type XVIII; alpha 1; Endostatin; KNO; KNO1

免疫原

E.coli, H1154-S1335

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156 pg/ml - 10000 pg/ml

基因名称

COL18A1

基因名全称

collagen, type XVIII, alpha 1

Uniprot ID

P39060

文献引用格式

Human Endostatin/COL18A1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0886)

背景资料

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

显色时间

25-30分钟

试剂盒内容

Catalog number	Description	Quantity
EK0886-CAP	96-well plate precoated with anti- human Endostatin antibody	1
EK0886-ST	Lyophilized recombinant human Endostatin standard	10ng/tube×2
EK0886-DA	Biotinylated anti- human Endostatin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	247	1523	5144	251	1462	5243
Standard deviation	14.07	109.65	216.04	14.3	130.11	319.82
CV(%)	5.7	7.2	4.2	5.7	8.9	6.1

典型数据示例

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.047	0.095	0.149	0.245	0.432	0.77	1.308	1.98

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Endostatin/COL18A1 ELISA Kit

EK0886

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

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检测方法

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实验时长

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