产品名称
Mouse Endostatin/COL18A1 ELISA Kit
指标别名
COL18A1; Collagen alpha 1(XVIII) chain; collagen; type XVIII; alpha 1; Endostatin; KNO; KNO1
免疫原
Expression system for standard: NS0; Immunogen sequence: H1591-K1774
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Collagen alpha-1(XVIII) chain
文献引用格式
Mouse Endostatin/COL18A1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1376)
背景资料
Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1376-CAP | 96-well plate precoated with anti- mouse Endostatin antibody | 1 |
| EK1376-ST | Lyophilized mouse Endostatin standard | 10ng/tube×2 |
| EK1376-DA | Biotinylated anti- mouse Endostatin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 306 | 1512 | 4729 | 308 | 1404 | 4765 |
| Standard deviation | 12.24 | 74.08 | 316.84 | 13.86 | 77.22 | 390.73 |
| CV(%) | 4% | 4.9% | 6.7% | 4.5% | 5.5% | 8.2% |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.011 | 0.042 | 0.073 | 0.129 | 0.247 | 0.534 | 1.147 | 2.004 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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