Mouse Endostatin/COL18A1 Quick ELISA Kit

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FEK1376

96T ￥3580
货期：现货

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Mouse Endostatin ELISA Kit standard curve
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产品简介>

产品名称

Mouse Endostatin/COL18A1 Quick ELISA Kit

规格/价格

96T|3580

指标别名

COL18A1; Collagen alpha 1(XVIII) chain; collagen; type XVIII; alpha 1; Endostatin; KNO; KNO1

免疫原

Expression system for standard: NS0; Immunogen sequence: H1591-K1774

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

156pg/ml-10000pg/ml

基因名称

Col18a1

基因名全称

collagen, type XVIII, alpha 1

Uniprot ID

P39061

文献引用格式

Mouse Endostatin/COL18A1 Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK1376)

背景资料

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

显色时间

15分钟

试剂盒内容

Catalog number	Description	Quantity
FEK1376-CAP	Anti-Pre-coated 96-well Strip Microplate	1
FEK1376-ST	Mouse Endostatin standard	10ng/tube×2
FEK1376-A	Mouse Endostatin Antibody A	3ml
FEK1376-B	Mouse Endostatin Antibody B	3ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

批内差/批间差

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	305	1523	4766	304	1462	4785
Standard deviation	12.2	74.63	314.56	13.07	74.56	282.32
CV(%)	4%	4.9%	6.6%	4.3%	5.1%	5.9%

典型数据示例

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.002	0.109	0.186	0.368	0.747	1.290	1.917	2.267

Mouse Endostatin/COL18A1 Quick ELISA Kit

FEK1376

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

应用范围

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检测方法

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Mouse Endostatin/COL18A1 Quick ELISA Kit

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