Monkey primate Thrombomodulin/THBD ELISA Kit

96T|2480

AHUS6; BDCA3; CD141; CD141 antigen; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin

Expression system for standard: NS0; Immunogen sequence: A19-S515

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

THBD

Thrombomodulin

P07204  

Monkey primate Thrombomodulin/THBD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0917-PR)

Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on monkey primate mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).

15-20分钟

Catalog number	Description	Quantity
EK0917-PR-CAP	Anti-Monkey THBD Pre-coated 96-well strip microplate	1
EK0917-PR-ST	Monkey THBD Standard	10ng/tube×2
EK0917-PR-DA	Monkey THBD Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	523	1074	2246	576	1187	2315
Standard deviation	24.1	40.1	92.1	47.2	86.7	180.6
CV (%)	4.6	3.7	4.1	8.2	7.3	7.8

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.032	0.095	0.151	0.27	0.48	0.913	1.521	2.097

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

monkey

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine