Mouse Thrombomodulin/THBD ELISA Kit

96T|2480

AHUS6; BDCA3; CD141; CD141 antigen; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin

Expression system for standard: NS0; Immunogen sequence: L17-S517

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

THBD

Thrombomodulin

P15306  

Mouse Thrombomodulin/THBD ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1119)

Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene (THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome (aHUS).

Catalog number	Description	Quantity
EK1119-CAP	96-well plate precoated with anti- mouse Thrombomodulin antibody	1
EK1119-ST	Lyophilized recombinant mouse Thrombomodulin standard	10ng/tube×2
EK1119-DA	Biotinylated anti- mouse Thrombomodulin antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	154	450	1390	169	408	1348
Standard deviation	6.46	33.3	55.6	9.46	36.31	60.66
CV(%)	4.2%	7.4%	4%	5.6%	8.9%	4.5%

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.019	0.068	0.104	0.219	0.38	0.769	1.299	1.957

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

mouse

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine

3.5h