Human Thrombomodulin/THBD Quick ELISA Kit

96T|3580

AHUS6; BDCA3; CD141; CD141 antigen; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin

Expression system for standard: NS0; Immunogen sequence: A19-S515

There is no detectable cross-reactivity with other relevant proteins.

62.5pg/ml-4000pg/ml

THBD

thrombomodulin

P07204  

Human Thrombomodulin/THBD Quick ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#FEK0917)

Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).

15分钟

Catalog number	Description	Quantity
FEK0917-CAP	Anti-Pre-coated 96-well Strip Microplate	1
FEK0917-ST	Human THBD standard	10ng/tube×2
FEK0917-A	Human THBD Antibody A	3ml
FEK0917-B	Human THBD Antibody B	3ml
AR1106-1	Sample diluent buffer	15ml
AR1106-7	TBS-T Wash Buffer (25x)	12ml
AR1104	TMB color developing agent	10ml
AR1105	Stop solution	10ml
PLA-SEA	Adhesive cover	2

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	164	418	1319	176	453	1243
Standard deviation	7.38	26.33	79.14	9.68	33.52	91.98
CV(%)	4.5%	6.3%	6%	5.5%	7.4%	7.4%

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.021	0.074	0.103	0.171	0.365	0.665	1.155	1.854

Store at 4℃ for 6 months.

ELISA

human

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA, citrate) and Urine