产品名称
Human Epiregulin/EREG ELISA Kit
指标别名
Proepiregulin; Epiregulin; EPR; EREG
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Human Epiregulin/EREG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1394)
背景资料
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1394-CAP | 96-well plate precoated with anti- human Epiregulin antibody | 1 |
| EK1394-ST | Lyophilized recombinant human Epiregulin standard | 10ng/tube×2 |
| EK1394-DA | Biotinylated anti- human Epiregulin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 3ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 317 | 1096 | 3978 | 307 | 1087 | 4172 |
| Standard deviation | 20.6 | 84.39 | 286.41 | 24.25 | 92.39 | 333.76 |
| CV(%) | 6.5 | 7.7 | 7.2 | 7.9 | 8.5 | 8 |
典型数据示例
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.014 | 0.073 | 0.138 | 0.277 | 0.501 | 0.87 | 1.686 | 2.529 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)