产品名称
Mouse Epiregulin/EREG ELISA Kit
指标别名
Proepiregulin; Epiregulin; EPR; EREG
免疫原
Expression system for standard: E.coli; Immunogen sequence: V53-L101
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Epiregulin/EREG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1395)
背景资料
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1395-CAP | 96-well plate precoated with anti- mouse Epiregulin antibody | 1 |
| EK1395-ST | Lyophilized recombinant mouse Epiregulin standard | 10ng/tube×2 |
| EK1395-DA | Biotinylated anti- mouse Epiregulin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 196 | 1010 | 4381 | 179 | 1069 | 4367 |
| Standard deviation | 11.76 | 65.65 | 227.81 | 13.96 | 73.76 | 231.45 |
| CV(%) | 6% | 6.5% | 5.2% | 7.8% | 6.9% | 5.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.005 | 0.019 | 0.047 | 0.085 | 0.178 | 0.41 | 0.879 | 1.813 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)