产品名称
Pig porcine Epiregulin/EREG ELISA Kit
指标别名
Proepiregulin; Epiregulin; EPR; EREG
免疫原
Expression system for standard: E.coli; Immunogen sequence: V60-L108
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Pig porcine Epiregulin/EREG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1394-PO)
背景资料
Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1394-PO-CAP | Anti-Pig EREG Pre-coated 96-well strip microplate | 1 |
| EK1394-PO-ST | Pig EREG Standard | 10ng/tube×2 |
| EK1394-PO-DA | Pig EREG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 26.2 | 88.7 | 144 | 25.4 | 91.5 | 151 |
| Standard deviation | 1.18 | 7.94 | 6.81 | 1.73 | 7.05 | 11.93 |
| CV (%) | 4.5 | 5.8 | 5.7 | 6.8 | 7.7 | 7.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.042 | 0.107 | 0.16 | 0.271 | 0.464 | 0.911 | 1.573 | 2.256 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)