产品名称
Mouse WIF1 ELISA Kit
指标别名
WIF 1; WIF1; WIF-1; WNT inhibitory factor 1
免疫原
Expression system for standard: NS0; Immunogen sequence: G29-W379
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Wnt inhibitory factor 1
文献引用格式
Mouse WIF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1523)
背景资料
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1523-CAP | 96-well plate precoated with anti- mouse WIF1 antibody | 1 |
| EK1523-ST | Lyophilized recombinant mouse WIF1 standard | 10ng/tube×2 |
| EK1523-DA | Biotinylated anti- mouse WIF1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 40 | 298 | 975 | 37 | 309 | 914 |
| Standard deviation | 1.92 | 22.94 | 39 | 1.88 | 28.73 | 43.87 |
| CV(%) | 4.8% | 7.7% | 4% | 5.1% | 9.3% | 4.8% |
典型数据示例
| Concentration (pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.05 | 0.123 | 0.2 | 0.304 | 0.606 | 1.029 | 1.732 | 2.436 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)