指标别名
WIF 1; WIF1; WIF-1; WNT inhibitory factor 1
免疫原
Expression system for standard: NS0; Immunogen sequence: G29-W379
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
WNT inhibitory factor 1
文献引用格式
Rat WIF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1564)
背景资料
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
试剂盒内容
| Catalog number | Description | Quantity |
| EK1564-CAP | 96-well plate precoated with anti- rat WIF1 antibody | 1 |
| EK1564-ST | Lyophilized recombinant rat WIF1 standard | 10ng/tube×2 |
| EK1564-DA | Biotinylated anti- rat WIF1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 46 | 179 | 730 | 48 | 172 | 703 |
| Standard deviation | 3.49 | 10.2 | 32.85 | 4.41 | 10.32 | 44.28 |
| CV(%) | 7.6% | 5.7% | 4.5% | 9.2% | 6% | 6.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.041 | 0.141 | 0.193 | 0.341 | 0.598 | 0.852 | 1.531 | 2.056 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)