产品名称
Human WIF1 ELISA Kit
指标别名
WIF 1; WIF1; WIF-1; WNT inhibitory factor 1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
23.4 pg/ml - 1500 pg/ml
基因名全称
WNT inhibitory factor 1
文献引用格式
Human WIF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1524)
背景资料
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1524-CAP | 96-well plate precoated with anti- human WIF1 antibody | 1 |
| EK1524-ST | Lyophilized recombinant human WIF1 standard | 10ng/tube×2 |
| EK1524-DA | Biotinylated anti- human WIF1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 43 | 210 | 939 | 40 | 222 | 983 |
| Standard deviation | 2.06 | 11.76 | 46.95 | 2.64 | 15.98 | 67.82 |
| CV(%) | 4.8 | 5.6 | 5 | 6.6 | 7.2 | 6.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.051 | 0.185 | 0.268 | 0.404 | 0.755 | 1.12 | 1.72 | 2.33 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)