Human Legumain/LGMN (total) ELISA Kit

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EK1566

96T ￥2480
货期：现货

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产品名称

Human Legumain/LGMN (total) ELISA Kit

规格/价格

96T|2480

指标别名

AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1

免疫原

NSO,V18-Y433

交叉反应

There is no detectable cross-reactivity with other relevant proteins.

检测范围

31.2 pg/ml - 2000 pg/ml

基因名称

LGMN

基因名全称

legumain

Uniprot ID

Q99538

文献引用格式

Human Legumain/LGMN (total) ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1566)

背景资料

Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.

显色时间

20-25分钟

试剂盒内容

Catalog number	Description	Quantity
EK1566-CAP	96-well plate precoated with anti- human Legumain(total) antibody	1
EK1566-ST	Lyophilized recombinant human Legumain(total) standard	10ng/tube×2
EK1566-DA	Biotinylated anti- human Legumain(total) antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	86	299	1057	90	289	1074
Standard deviation	5.93	23.62	45.45	6.21	26.58	54.77
CV(%)	6.9	7.9	4.3	6.9	9.2	5.1

典型数据示例

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.009	0.034	0.065	0.119	0.223	0.479	0.912	1.873

保存条件与有效期

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Human Legumain/LGMN (total) ELISA Kit

EK1566

产品简介>

产品名称

规格/价格

指标别名

免疫原

交叉反应

检测范围

基因名称

基因名全称

Uniprot ID

文献引用格式

背景资料

显色时间

试剂盒内容

批内差/批间差

典型数据示例

保存条件与有效期

检验物种

检测方法

样本类型

实验时长

其他介绍>

引用文献(0)>

All ELISA(1) All Species Human(1) 清除

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