产品名称
Rat Legumain/LGMN ELISA Kit
指标别名
AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1
免疫原
Expression system for standard: NS0; Immunogen sequence: V18-N325
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Rat Legumain/LGMN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2100)
背景资料
Legumain is a protein that in humans is encoded by the LGMN gene. It is mapped to 6q32. This gene encodes a cysteine protease, legumain, that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
试剂盒内容
| Catalog number | Description | Quantity |
| EK2100-CAP | 96-well plate precoated with anti- rat Legumain antibody | 1 |
| EK2100-ST | Lyophilized recombinant rat Legumain standard | 10ng/tube×2 |
| EK2100-DA | Biotinylated anti- rat Legumain antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 35 | 212 | 806 | 52 | 234 | 939 |
| Standard deviation | 1.72 | 10.81 | 41.91 | 3.43 | 13.57 | 68.55 |
| CV(%) | 4.9% | 5.1% | 5.2% | 6.6% | 5.8% | 7.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.021 | 0.092 | 0.15 | 0.266 | 0.483 | 0.838 | 1.51 | 2.624 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)
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