产品名称
Mouse Legumain/LGMN ELISA Kit
指标别名
AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1
免疫原
Expression system for standard: NS0; Immunogen sequence: V18-Y435
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse Legumain/LGMN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1567)
背景资料
Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1567-CAP | 96-well plate precoated with anti- mouse Legumain(total) antibody | 1 |
| EK1567-ST | Lyophilized recombinant mouse Legumain(total) standard | 10ng/tube×2 |
| EK1567-DA | Biotinylated anti- mouse Legumain(total) antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 84 | 270 | 1001 | 90 | 264 | 954 |
| Standard deviation | 3.94 | 12.42 | 68.06 | 4.32 | 15.31 | 65.82 |
| CV(%) | 4.7% | 4.6% | 6.8% | 4.8% | 5.8% | 6.9% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.038 | 0.178 | 0.262 | 0.394 | 0.615 | 0.962 | 1.45 | 2.492 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)