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Mouse Legumain/LGMN ELISA Kit

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EK1567

  • 96T ¥2480
  • 货期: 现货
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产品简介>

产品名称
Mouse Legumain/LGMN ELISA Kit
规格/价格
96T|2480
指标别名
AEP; cysteine 1; Legumain; LGMN; LGMN1; PRSC1
免疫原
Expression system for standard: NS0; Immunogen sequence: V18-Y435
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2pg/ml-2000pg/ml
基因名称
Lgmn
基因名全称
Legumain
Uniprot ID
O89017  
文献引用格式
Mouse Legumain/LGMN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1567)
背景资料
Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.
试剂盒内容
Catalog numberDescriptionQuantity
EK1567-CAP96-well plate precoated with anti- mouse Legumain(total) antibody1
EK1567-STLyophilized recombinant mouse Legumain(total) standard10ng/tube×2
EK1567-DABiotinylated anti- mouse Legumain(total) antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)84270100190264954
Standard deviation3.9412.4268.064.3215.3165.82
CV(%)4.7%4.6%6.8%4.8%5.8%6.9%
典型数据示例
Concentration (pg/ml)031.262.512525050010002000
O.D.0.0380.1780.2620.3940.6150.9621.452.492
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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