产品名称
Human Vitamin D binding protein/GC ELISA Kit
指标别名
DBP; DBP/GC; GC; Gc globulin; Group specific component; VDB; VDBG; VDBP; Vitamin D binding protein
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
GC, vitamin D binding protein
文献引用格式
Human Vitamin D binding protein/GC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1641)
背景资料
Vitamin D-binding protein, also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene.The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1641-CAP | 96-well plate precoated with anti- human Vitamin DBP antibody | 1 |
| EK1641-ST | Lyophilized recombinant human Vitamin DBP standard | 10ng/tube×2 |
| EK1641-DA | Biotinylated anti- human Vitamin DBP antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 294 | 1526 | 5228 | 313 | 1484 | 5355 |
| Standard deviation | 17.34 | 97.66 | 245.71 | 22.22 | 123.17 | 348.07 |
| CV(%) | 5.9 | 6.4 | 4.7 | 7.1 | 8.3 | 6.5 |
典型数据示例
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.034 | 0.211 | 0.283 | 0.373 | 0.611 | 1.025 | 1.622 | 2.382 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk