产品名称
Mouse Vitamin D binding protein/GC ELISA Kit
指标别名
DBP; DBP/GC; GC; Gc globulin; Group specific component; VDB; VDBG; VDBP; Vitamin D binding protein
免疫原
Expression system for standard: NS0; Immunogen sequence: L17-S476
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
3.12 ng/ml - 200 ng/ml
基因名全称
GC, vitamin D binding protein
文献引用格式
Mouse Vitamin D binding protein/GC ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK2063)
背景资料
Vitamin D-binding protein (DBP), also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. It is mapped to 5 E1; 5 44.32 cM. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK2063-CAP | 96-well plate precoated with anti- mouse Vitamin DBP antibody | 1 |
| EK2063-ST | Lyophilized recombinant mouse Vitamin DBP standard | 200ng/tube×2 |
| EK2063-DA | Biotinylated anti- mouse Vitamin DBP antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 4834 | 16968 | 53969 | 5556 | 14450 | 78200 |
| Standard deviation | 261.52 | 385.17 | 2147.97 | 272.8 | 1079.42 | 2697.9 |
| CV(%) | 5.4% | 2.3% | 4% | 4.9% | 7.5% | 3.5% |
典型数据示例
| Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.038 | 0.061 | 0.070 | 0.106 | 0.207 | 0.456 | 1.035 | 1.855 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, cell lysates, serum, plasma (heparin) and urine.