Anti-FAK/PTK2 Antibody (Clone#OTI4A8)

Mouse monoclonal antibody

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M00151-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and PTK2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTK2 monoclonal antibody M00151-1, (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)
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Immunofluorescent staining of HeLa cells using anti-PTK2 mouse monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)
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Anti-PTK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK2 .
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK2(Cat# M00151-1).
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Western blot analysis of extracts (35ug) from 9 different cell by using anti-PTK2 monoclonal antibody (1:500).
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产品简介>

产品名称

Anti-FAK/PTK2 Antibody (Clone#OTI4A8)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

FADK; FADK 1; FAK; FAK1; Focal adhesion kinase 1; pp125FAK; Protein tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

PTK2

克隆号

OTI4A8

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human PTK2(NP_722560) produced in HEK293T cell.

计算分子量

119 kDa

实际分子量

119 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Uniprot ID

Q05397

文献引用格式

FAK/PTK2 Antibody (Clone#OTI4A8) (Boster Biological Technology, Wuhan, China. Catalog#M00151-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

PTK2 protein tyrosine kinase 2

蛋白名全称

Focal adhesion kinase 1(FADK 1)

实验方案>

实验步骤

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