Anti-FAK/PTK2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0662

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-PTK2 antibody (PB0662). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
Lane 3: human K562 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: rat brain tissue lysates,
Lane 8: rat C6 whole cell lysates,
Lane 9: mouse lung tissue lysates,
Lane 10: mouse brain tissue lysates,
Lane 11: mouse RAW264.7 whole cell lysates,
Lane 12: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PTK2 antigen affinity purified polyclonal antibody (PB0662) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PTK2 at approximately 125 kDa. The expected band size for PTK2 is at 119 kDa.
all(2)
IHC analysis of FAK/PTK2 using anti-FAK/PTK2 antibody (PB0662).
FAK/PTK2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-FAK/PTK2 Antibody (PB0662) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-FAK/PTK2 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

FADK; FADK 1; FAK; FAK1; Focal adhesion kinase 1; pp125FAK; Protein tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

PTK2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human PTK2 identical to the related mouse and rat sequences.

计算分子量

119 kDa

实际分子量

119 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Uniprot ID

Q05397

文献引用格式

FAK/PTK2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0662)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

PTK2 protein tyrosine kinase 2

蛋白名全称

Focal adhesion kinase 1

实验方案>

实验步骤

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