| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-200 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
| ImmunoPrecipitation (IP): | 1:30 |
| Flow Cytometry (FCM): | 1:50 |

Western blot analysis of anti-FAK/PTK2 antibody (BM4303). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAK/PTK2 antigen affinity purified monoclonal antibody (BM4303) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAK/PTK2 at approximately 119 kDa. The expected band size for FAK/PTK2 is at 119 kDa.

Immunohistochemical analysis of paraffin-embedded mouse spleen, using FAK Antibody.

Immunofluorescent analysis of Hela cells, using FAK Antibody.

Western blot analysis of anti-FAK/PTK2 antibody (BM4303). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAK/PTK2 antigen affinity purified monoclonal antibody (BM4303) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAK/PTK2 at approximately 119 kDa. The expected band size for FAK/PTK2 is at 119 kDa.

Immunohistochemical analysis of paraffin-embedded mouse spleen, using FAK Antibody.

Immunofluorescent analysis of Hela cells, using FAK Antibody.






