Flow cytometric analysis of living 293T cells transfected with CD86 overexpression plasmid , Red) using anti-CD86 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
all(5)
Flow cytometric analysis of living Raji cells, using anti-CD86 antibody, compared to an isotype control (green) (1:100).
all(5) 
Flow cytometric analysis of living Ramos cells, using anti-CD86 antibody, compared to an isotype control (green) (1:100).
all(5) 
Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD86 monoclonal antibody (1:500).
all(5) 
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD86 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD86 (1:1000).
all(5) | Western blot (WB): | 1:500~1000 |
| Flow cytometry (FCM): | 1:100 |
Flow cytometric analysis of living 293T cells transfected with CD86 overexpression plasmid , Red) using anti-CD86 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
Flow cytometric analysis of living Raji cells, using anti-CD86 antibody, compared to an isotype control (green) (1:100).
Flow cytometric analysis of living Ramos cells, using anti-CD86 antibody, compared to an isotype control (green) (1:100).
Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD86 monoclonal antibody (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD86 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD86 (1:1000).
Flow cytometric analysis of living 293T cells transfected with CD86 overexpression plasmid , Red) using anti-CD86 antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
Flow cytometric analysis of living Raji cells, using anti-CD86 antibody, compared to an isotype control (green) (1:100).
Flow cytometric analysis of living Ramos cells, using anti-CD86 antibody, compared to an isotype control (green) (1:100).
Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD86 monoclonal antibody (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD86 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD86 (1:1000).
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