Anti-B7-2/CD86 Antibody (Clone#BFF-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP FCM

BM4121

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-CD86 antibody (BM4121). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Daudi whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse RAW264.7 whole cell lysates,
Lane 7: mouse A20 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD86 antigen affinity purified monoclonal antibody (BM4121) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD86 at approximately 72 kDa. The expected band size for CD86 is at 38 kDa.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody .
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Immunofluorescent analysis of K562 cells, using CD86 Antibody .
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产品简介>

产品名称

Anti-B7-2/CD86 Antibody (Clone#BFF-3)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Activation B7 2 antigen; B7 2; B70; BU63; CD28LG2; CD86; CD86 molecule; FUN 1; LAB72

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP, FCM

基因名称

CD86

克隆号

BFF-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human CD86

计算分子量

38 kDa

实际分子量

60-80 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD86 is officially called Cluster of Differentiation 86, also known as B7-2. Being a member of the immunoglobulin superfamily, it is a protein expressed on antigen-presenting cells. The CD86 gene is mapped to human chromosome 3q21. The smallest transcript, 828 bp, which the authors termed CD86deltaTM, has a deletion from nucleotide 686 to nucleotide 829(i.e., exon 6) and encodes a 275-amino acid protein. CD86deltaTM enhances proliferation and cytokine production by both naive and memory T cells, providing costimulatory signals necessary for T cell activation and survival.

Uniprot ID

P42081

文献引用格式

B7-2/CD86 Antibody (Clone#BFF-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4121)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

T-lymphocyte activation antigen CD86

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实验步骤

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