Anti-B7-2/CD86 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9251

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-B7-2/CD86 antibody (PB9251). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B7-2/CD86 antigen affinity purified polyclonal antibody (PB9251) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B7-2/CD86 at approximately 60-80 kDa. The expected band size for B7-2/CD86 is at 38 kDa.
all(3)
Western blot analysis of anti-B7-2/CD86 antibody (PB9251). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: mouse J774A.1 whole cell lysates,
Lane 4: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B7-2/CD86 antigen affinity purified polyclonal antibody (PB9251) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B7-2/CD86 at approximately 60-80 kDa. The expected band size for B7-2/CD86 is at 38 kDa.
all(3)
IHC analysis of B7-2/CD86 using anti-B7-2/CD86 antibody (PB9251).
B7-2/CD86 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-B7-2/CD86 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

Activation B7 2 antigen; B7 2; B70; BU63; CD28LG2; CD86; CD86 molecule; FUN 1; LAB72

产品类型

Polyclonal

检验物种

human, mouse

应用范围

WB, IHC

基因名称

CD86

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86.

计算分子量

38 kDa

实际分子量

60-80 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Uniprot ID

P42081

RRID

AB_3082036

文献引用格式

B7-2/CD86 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9251)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD86 molecule

蛋白名全称

T-lymphocyte activation antigen CD86

实验方案>

实验步骤

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