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Anti-SOD1 Antibody (Clone#OTI8B10)

Mouse monoclonal antibody

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筛选器: All WB IHC FCM

M00238-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Western blot analysis of anti-SOD1 antibody (M00238-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human HepG2 whole cell lysates,
    Lane 2: human Hela whole cell lysates,
    Lane 3: human A549 whole cell lysates,
    Lane 4: human MCF-7 whole cell lysates,
    Lane 5: rat brain tissue lysates,
    Lane 6: rat spleen tissue lysates,
    Lane 7: mouse brain tissue lysates,
    Lane 8: mouse spleen tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-SOD1 antigen affinity purified monoclonal antibody (M00238-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.

    all(5)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00238-1)

    all(5)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00238-1)

    all(5)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SOD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00238-1)

    all(5)
  • HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SOD1 antibody, and then analyzed by flow cytometry.

    all(5)

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产品名称
Anti-SOD1 Antibody (Clone#OTI8B10)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
ALS; ALS1; homodimer; IPOA; SOD; SOD1; Superoxide dismutase [Cu Zn]
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, FCM
基因名称
SOD1
克隆号
OTI8B10
宿主
Mouse
抗体亚型
IgG2b
免疫原
Full length human recombinant protein of human SOD1 (NP_000445) produced in HEK293T cell.
计算分子量
16 kDa
实际分子量
16-18 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]
Uniprot ID
P00441  
文献引用格式
SOD1 Antibody (Clone#OTI8B10) (Boster Biological Technology, Wuhan, China. Catalog#M00238-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Superoxide dismutase [Cu-Zn]
推荐稀释比
Western blot (WB):1:1000~2000
Immunohistochemistry (IHC):1:50
Flow cytometry (FCM):1:100

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    Anti-SOD1 Antibody (Clone#OTI8B10)

    筛选器: All WB IHC FCM

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