Anti-SOD1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA1401

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of SOD1 using anti-SOD1 antibody (BA1401). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human hepg2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: Rat liver tissue lysates,
Lane 5: Mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (BA1401) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD1 at approximately 18 kDa. The expected band size for SOD1 is at 16 kDa.
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IHC analysis of SOD1 using anti-SOD1 antibody (BA1401).
SOD1 was detected in a paraffin-embedded section of rat kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOD1 Antibody (BA1401) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of SOD1 using anti-SOD1 antibody (BA1401).
SOD1 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOD1 Antibody (BA1401) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of SOD1 using anti-SOD1 antibody (BA1401).
SOD1 was detected in a paraffin-embedded section of rat small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOD1 Antibody (BA1401) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of SOD1 using anti-SOD1 antibody (BA1401).
SOD1 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOD1 Antibody (BA1401) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IHC analysis of SOD1 using anti-SOD1 antibody (BA1401).
SOD1 was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SOD1 Antibody (BA1401) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(7)
IF analysis of SOD1 using anti-SOD1 antibody (BA1401).
SOD1 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-SOD1 Antibody (BA1401) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
all(7)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SOD1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ALS; ALS1; homodimer; IPOA; SOD; SOD1; Superoxide dismutase [Cu Zn]

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

SOD1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1, different from the mouse sequence by two amino acids.

计算分子量

16 kDa

实际分子量

18 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Superoxide dismutases(SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

Uniprot ID

P00441

文献引用格式

SOD1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1401)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

superoxide dismutase 1, soluble

蛋白名全称

Superoxide dismutase[Cu-Zn]

实验方案>

实验步骤

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