Anti-SOD1 Antibody (Clone#AOEI-19)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF FCM

BM4934

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-SOD1 antibody (BM4934). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SOD1 antigen affinity purified monoclonal antibody (BM4934) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SOD1 at approximately 18 kDa. The expected band size for SOD1 is at 16 kDa.
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IHC analysis of SOD1 using anti-SOD1 antibody (BM4934) .
SOD1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-SOD1 Antibody (BM4934) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-SOD1 Antibody (Clone#AOEI-19)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ALS; ALS1; homodimer; IPOA; SOD; SOD1; Superoxide dismutase [Cu Zn]

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

SOD1

克隆号

AOEI-19

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human SOD1

计算分子量

16 kDa

实际分子量

18 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

Uniprot ID

P00441

文献引用格式

SOD1 Antibody (Clone#AOEI-19) (Boster Biological Technology, Wuhan, China. Catalog#BM4934)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Superoxide dismutase [Cu-Zn]

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实验步骤

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