| Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:150 |

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00356-4)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00356-4)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00356-4)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CEACAM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CEACAM5.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00356-4)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00356-4)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CEACAM5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, M00356-4)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CEACAM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CEACAM5.



