Anti-CD18/ITGB2 Antibody (Clone#1A3/2A10)

Mouse monoclonal antibody

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筛选器： All IHC WB

M00458-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (M00458-1).
CD18/ITGB2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CD18/ITGB2 Antibody (M00458-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (M00458-1).
CD18/ITGB2 was detected in a paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-CD18/ITGB2 Antibody (M00458-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(3)
Western blot analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (M00458-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-CD18/ITGB2 antigen affinity purified monoclonal antibody (M00458-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD18/ITGB2 at approximately 90-100 kDa. The expected band size for CD18/ITGB2 is at 85 kDa.
all(3)

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产品简介>

产品名称

Anti-CD18/ITGB2 Antibody (Clone#1A3/2A10)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD18; Integrin beta 2; ITGB2; LAD; LCAMB; LFA 1; MAC 1; MF17; MFI7

产品类型

Monoclonal

检验物种

human

应用范围

IHC, WB

基因名称

ITGB2

克隆号

1A3/2A10

宿主

Mouse

抗体亚型

IgG1

免疫原

E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18.

计算分子量

85 kDa

实际分子量

90-100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ITGB2(INTEGRIN, BETA-2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.

Uniprot ID

P05107

RRID

AB_3081778

文献引用格式

CD18/ITGB2 Antibody (Clone#1A3/2A10) (Boster Biological Technology, Wuhan, China. Catalog#M00458-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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