Anti-CD18/ITGB2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF

PB9141

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti- ITGB2 antibody (PB9141). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- ITGB2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ITGB2 at approximately 90-100KD. The expected band size for ITGB2 is at 85KD.
all(4)
IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141).
CD18/ITGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-CD18/ITGB2 Antibody (PB9141) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141).
CD18/ITGB2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-CD18/ITGB2 Antibody (PB9141) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis using anti- ITGB2 antibody (PB9141). detected in paraffin-embedded section of human tonsil tissue. The tissue section were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(4)

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产品简介>

产品名称

Anti-CD18/ITGB2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD18; Integrin beta 2; ITGB2; LAD; LCAMB; LFA 1; MAC 1; MF17; MFI7

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, IF

基因名称

ITGB2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD18 recombinant protein (Position: Q404-S769). Human CD18 shares 76% amino acid (aa) sequence identity with mouse CD18.

计算分子量

85 kDa

实际分子量

90-100 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ITGB2(INTEGRIN, BETA-2), also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. ITGB2 is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated CD18. The ITGB2 gene is mapped to 21q22.3. The expression of CD18 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of ITGB2 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although ITGB2 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.

Uniprot ID

P05107

RRID

AB_3081987

文献引用格式

CD18/ITGB2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9141)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)

蛋白名全称

Integrin beta-2

实验方案>

实验步骤

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