| Western blot (WB): | 1:2000 |
| Immunohistochemistry (IHC): | 1:50 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:100 |

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00575-2)

Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX .

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LOX monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00575-2)

Anti-LOX mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX .



