Western blot analysis of UPF1 using anti-UPF1 antibody (M00900). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Mouse brain tissue lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human HELA whole cell lysates,
Lane 8: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-UPF1 antigen affinity purified monoclonal antibody (M00900) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:100. Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of UPF1 using anti-UPF1 antibody (M00900). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Mouse brain tissue lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human HELA whole cell lysates,
Lane 8: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-UPF1 antigen affinity purified monoclonal antibody (M00900) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:100. Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Western blot analysis of UPF1 using anti-UPF1 antibody (M00900). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Mouse brain tissue lysates,
Lane 3: Mouse RAW264.7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human Raji whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human HELA whole cell lysates,
Lane 8: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-UPF1 antigen affinity purified monoclonal antibody (M00900) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of UPF1 using anti-UPF1 antibody (M00900).
UPF1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with mouse anti-UPF1 Antibody (M00900) at a dilution of 1:100. Dylight594-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1141) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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