Western blot analysis of UPF1 using anti-UPF1 antibody (PB0862). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: SK-OV-3 whole cell lysates,
Lane 5: PC-3 whole cell lysates,
Lane 6: HEK293 whole cell lysates,
Lane 7: RH35 whole cell lysates,
Lane 8: HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody (PB0862) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of PC-3 cells using anti-UPF1 antibody (PB0862).
Overlay histogram showing PC-3 cells stained with PB0862 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UPF1 Antibody (PB0862) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of RENT1/hUPF1 using anti- RENT1/hUPF1 antibody (PB0862).RENT1/hUPF1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- RENT1/hUPF1 Antibody (PB0862) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
all(6) | Western blot (WB): | 1:1000-5000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of UPF1 using anti-UPF1 antibody (PB0862). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: SK-OV-3 whole cell lysates,
Lane 5: PC-3 whole cell lysates,
Lane 6: HEK293 whole cell lysates,
Lane 7: RH35 whole cell lysates,
Lane 8: HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody (PB0862) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of PC-3 cells using anti-UPF1 antibody (PB0862).
Overlay histogram showing PC-3 cells stained with PB0862 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UPF1 Antibody (PB0862) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of RENT1/hUPF1 using anti- RENT1/hUPF1 antibody (PB0862).RENT1/hUPF1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- RENT1/hUPF1 Antibody (PB0862) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot analysis of UPF1 using anti-UPF1 antibody (PB0862). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Raji whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: SK-OV-3 whole cell lysates,
Lane 5: PC-3 whole cell lysates,
Lane 6: HEK293 whole cell lysates,
Lane 7: RH35 whole cell lysates,
Lane 8: HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody (PB0862) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of UPF1 using anti-UPF1 antibody (PB0862).
UPF1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-UPF1 Antibody (PB0862) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Flow Cytometry analysis of PC-3 cells using anti-UPF1 antibody (PB0862).
Overlay histogram showing PC-3 cells stained with PB0862 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UPF1 Antibody (PB0862) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of RENT1/hUPF1 using anti- RENT1/hUPF1 antibody (PB0862).RENT1/hUPF1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- RENT1/hUPF1 Antibody (PB0862) overnight at 4°C. DyLight 488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
联系我们027-67845390
关注我们
本司产品仅用于科研，不用于临床诊断和治疗
联系方式：027-67845390/1/2 技术支持：武汉丰网
© 1993-2025 Boster Biological Technology co.Itd E-mail:boster@boster.com
鄂ICP备05005548号-2
鄂公网安备 42018502007312号
积分商城 
购物车 
登录/注册 
您当前的位置： 
说明书
一键复制产品信息
成功添加到购物车
微信客服
微信扫一扫立即咨询
