Western blot analysis of anti-Cytokeratin 8/KRT8 antibody (M01421-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytokeratin 8/KRT8 antigen affinity purified monoclonal antibody (M01421-3) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 8/KRT8 at approximately 54 kDa. The expected band size for Cytokeratin 8/KRT8 is at 54 kDa.
IHC analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with mouse anti-Cytokeratin 8/KRT8 Antibody (M01421-3) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human intestinal cancer tissue. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human mammary cancer tissue. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in an immunocytochemical section of U2OS cells. Biotin Conjugated Goat anti-Mouse IgG (BA1001) was used as secondary antibody. The section was developed using Cy3 Conjugated Avidin (BA1037).
Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Overlay histogram showing A549 cells stained with A04887-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 8/KRT8 Antibody ((M01421-3, 1:100). DyLight®488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG (Catalog # BA1046) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of anti-Cytokeratin 8/KRT8 antibody (M01421-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytokeratin 8/KRT8 antigen affinity purified monoclonal antibody (M01421-3) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 8/KRT8 at approximately 54 kDa. The expected band size for Cytokeratin 8/KRT8 is at 54 kDa.
IHC analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with mouse anti-Cytokeratin 8/KRT8 Antibody (M01421-3) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human intestinal cancer tissue. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human mammary cancer tissue. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in an immunocytochemical section of U2OS cells. Biotin Conjugated Goat anti-Mouse IgG (BA1001) was used as secondary antibody. The section was developed using Cy3 Conjugated Avidin (BA1037).
Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Overlay histogram showing A549 cells stained with A04887-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 8/KRT8 Antibody ((M01421-3, 1:100). DyLight®488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG (Catalog # BA1046) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Western blot analysis of anti-Cytokeratin 8/KRT8 antibody (M01421-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human HEK293 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytokeratin 8/KRT8 antigen affinity purified monoclonal antibody (M01421-3) and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 8/KRT8 at approximately 54 kDa. The expected band size for Cytokeratin 8/KRT8 is at 54 kDa.
IHC analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was incubated with mouse anti-Cytokeratin 8/KRT8 Antibody (M01421-3) at a dilution of 1:200 and developed using HRP Conjugated mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human intestinal cancer tissue. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody.The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in a paraffin-embedded section of human mammary cancer tissue. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IF analysis of Cytokeratin 8/KRT8 using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Cytokeratin 8/KRT8 was detected in an immunocytochemical section of U2OS cells. Biotin Conjugated Goat anti-Mouse IgG (BA1001) was used as secondary antibody. The section was developed using Cy3 Conjugated Avidin (BA1037).
Flow Cytometry analysis of A549 cells using anti-Cytokeratin 8/KRT8 antibody (M01421-3).
Overlay histogram showing A549 cells stained with A04887-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 8/KRT8 Antibody ((M01421-3, 1:100). DyLight®488 conjugated goat anti-mouse IgG (BA1126, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG (Catalog # BA1046) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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