Anti-Cytokeratin 8/KRT8 Antibody (Clone#OTI1B9)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF

M01421-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-Cytokeratin 8/KRT8 antibody (M01421-5). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytokeratin 8/KRT8 antigen affinity purified monoclonal antibody (M01421-5) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 8/KRT8 at approximately 50 kDa. The expected band size for Cytokeratin 8/KRT8 is at 54 kDa.
all(2)
Anti-KRT8 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 .
all(2)

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产品名称

Anti-Cytokeratin 8/KRT8 Antibody (Clone#OTI1B9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CARD2; Cell and organelle markers; CK 8; CK8; CYK8; Cytokeratin 8; Cytoskeleton Marker; K2C8; K8; keratin 8; KO; KRT8; Type II keratin Kb8

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

WB, ICC/IF

基因名称

KRT8

克隆号

OTI1B9

宿主

Mouse

抗体亚型

IgG2a

免疫原

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

计算分子量

54 kDa

实际分子量

50 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Uniprot ID

P05787

文献引用格式

Cytokeratin 8/KRT8 Antibody (Clone#OTI1B9) (Boster Biological Technology, Wuhan, China. Catalog#M01421-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Keratin, type II cytoskeletal 8

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实验步骤

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