Anti-p70 S6K/RPS6KB1 Antibody (Clone#OTI1G4)

Mouse monoclonal antibody

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M01475-5

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01475-5)
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPS6KB1 antibody, and then analyzed by flow cytometry.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01475-5)
all(8)
Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1 .
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RPS6KB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01475-5)
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Flow cytometric Analysis of Jurkat cells, using anti-RPS6KB1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
all(8)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
all(8)

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产品简介>

产品名称

Anti-p70 S6K/RPS6KB1 Antibody (Clone#OTI1G4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

p70 alpha; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K 1; p70 S6K alpha; p70 S6KA; p70(S6K); p70(S6K) alpha; p70S6K; P70S6K1; PS6K; Ribosomal protein S6 kinase I; RPS6KB1; S6K; S6K beta 1; S6K1; STK14A

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, FCM

基因名称

RPS6KB1

克隆号

OTI1G4

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.

计算分子量

59 kDa

实际分子量

59 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq]

Uniprot ID

P23443

文献引用格式

p70 S6K/RPS6KB1 Antibody (Clone#OTI1G4) (Boster Biological Technology, Wuhan, China. Catalog#M01475-5)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ribosomal protein S6 kinase B1

蛋白名全称

Ribosomal protein S6 kinase beta-1

实验方案>

实验步骤

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