Anti-p70 S6K/RPS6KB1 (Phospho-T421+S424) Antibody (Clone#BHD-18)

Rabbit Monoclonal antibody

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筛选器： All WB IP

BM4141

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of anti-p70 S6K/RPS6KB1 (Phospho-T421+S424) antibody (BM4141). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p70 S6K/RPS6KB1 (Phospho-T421+S424) antigen affinity purified monoclonal antibody (BM4141) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for p70 S6K/RPS6KB1 (Phospho-T421+S424) at approximately 270 kDa. The expected band size for p70 S6K/RPS6KB1 (Phospho-T421+S424) is at 242 kDa.
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产品名称

Anti-p70 S6K/RPS6KB1 (Phospho-T421+S424) Antibody (Clone#BHD-18)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

p70 alpha; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K 1; p70 S6K alpha; p70 S6KA; p70(S6K); p70(S6K) alpha; p70S6K; P70S6K1; PS6K; Ribosomal protein S6 kinase I; RPS6KB1; S6K; S6K beta 1; S6K1; STK14A

产品类型

Monoclonal

检验物种

human, rat

应用范围

WB, IP

基因名称

RPS6KB1

克隆号

BHD-18

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Phospho-S6K1 (T421 + S424)

计算分子量

59 kDa

实际分子量

59 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Uniprot ID

P23443

文献引用格式

p70 S6K/RPS6KB1 (Phospho-T421+S424) Antibody (Clone#BHD-18) (Boster Biological Technology, Wuhan, China. Catalog#BM4141)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Ribosomal protein S6 kinase beta-1

实验方案>

实验步骤

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