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Anti-BubR1/BUB1B Antibody (Clone#OTI5D9)

Mouse monoclonal antibody

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M01564-4

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Anti-BUB1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B .

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M01564-4, Dilution 1:50)

    all(13)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B(Cat# M01564-4).

    all(13)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-BubR1/BUB1B Antibody (Clone#OTI5D9)
规格/价格
50μl|1280 100μl|2180 150μl|2800
指标别称
Bub1A; BUB1B; BUB1beta; BubR1; hBUBR1; MAD3L; Protein SSK1; SSK1
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF
基因名称
BUB1B
克隆号
OTI5D9
宿主
Mouse
抗体亚型
IgG1
免疫原
Full-length protein expressed in 293T cell transfected with human BUB1B expression vector
计算分子量
120 kDa
实际分子量
119.5 kDa
成分
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Uniprot ID
O60566  
文献引用格式
BubR1/BUB1B Antibody (Clone#OTI5D9) (Boster Biological Technology, Wuhan, China. Catalog#M01564-4)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
BUB1 mitotic checkpoint serine/threonine kinase B
蛋白名全称
Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
推荐稀释比
Western blot (WB):1:2000
Immunohistochemistry (IHC):1:50
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100

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